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pECTO金黃色葡萄球菌染色體基因異位互補(bǔ)質(zhì)粒 貨號：P0434

信息

別名 金黃葡萄球染體異位互補(bǔ)
原核抗性 Amp
克隆菌株 DH5α
培養(yǎng)條件 37℃
質(zhì)粒宿主 金黃色葡萄球菌
質(zhì)粒用途 蛋白表達(dá)
原核抗性 氨芐青霉素

簡介

          pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureu.pECTO a Gram negative/Gram positive shuttle vector derived from pMAD2 designed to integrate DNA sequences between SAOUHSC_00278 and SAOUHSC_00279 (NCTC8325 nomenclature HG003). It carries about 1kb of both sequences. A terminator introduced between the SAOUHSC_00278 and SAOUHSC_00279 sequences prevents polar effects associated with the expression of inserted genes.This a Gram negative/Gram positive shuttle vector with two specialized replicons and antibiotic markers. pBR322 replicon and ampicillin resistance for E. coli. pE194(ts) replicon and erythromycin resistance for S. aureus. In S. aureus, the plasmid propagates at T<30°C, it becomes a suicide vector at T>37°C.

圖譜


garmin 230